Asparagine Peptide Lyases

Immobilisation of hydroxynitrile lyases.

Hydroxynitrile lyases are a versatile group of enzymes that are applied both in the laboratory and on an industrial scale. What makes them particularly interesting is that to date five structurally unrelated categories of hydroxynitrile lyases have been discovered. Given their great importance they have often been immobilised utilising many different methodologies. Therefore the hydroxynitrile ...

2 Lyases JASSEM G . MAHDI

An antigenic peptide produced by reverse splicing and double asparagine deamidation.

A variety of unconventional translational and posttranslational mechanisms contribute to the production of antigenic peptides, thereby increasing the diversity of the peptide repertoire presented by MHC class I molecules. Here, we describe a class I-restricted peptide that combines several posttranslational modifications. It is derived from tyrosinase and recognized by tumor-infiltrating lympho...

Active-site-directed inhibition of asparagine N-glycosyltransferases with epoxy-peptide derivatives.

The hexapeptide Arg-Asn-Gly-epoxyethylglycine-Ala-Val-OMe specifically inactivates membrane-bound N-glycosyltransferases. The specificity is demonstrated by the inability of peptides containing 2,3-epoxypropyl-, allyl- and vinyl-glycine in the epoxyethylglycine position to function as inhibitors. The inhibition is concentration-dependent and follows first-order kinetics, but requires disruption...

Pectic Acid Lyases of Bacillus polymyxa 1

Four enzymes were separated from an extracellular preparation of Bacillus polymyxa by carboxymethylcellulose column chromatography. The pH optima were 8.3 to 8.5, 8.7 to 8.9, 9.2 to 9.4, and 9.5 to 9.6. All of the enzymes required calcium ion for maximum activity, whereas strontium ion was only partially effective in stimulating activity. Cobalt was the only other cation tested which was effect...